|国家预印本平台
首页|Dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis

Dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis

Dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis

来源:medRxiv_logomedRxiv
英文摘要

Abstract BackgroundThe diagnosis of Human African Trypanosomiasis (HAT) typically involves two steps: a serological screen, followed by the detection of living trypanosome parasites in the blood or lymph node aspirate. Live parasites can, however, remain undetected in some seropositive individuals, who we hypothesize are infected with Trypanosoma brucei gambiense parasites in their extravascular dermis. Methods and findingsTo test this hypothesis, we conducted a prospective observational cohort study in the gambiense HAT (gHAT) focus of Forecariah, in the Republic of Guinea. 5,417 subjects in this disease foci underwent serological screening for gHAT. Of these individuals, 66 were enrolled into our study, of whom 40 were seronegative, 8 were seropositive but unconfirmed, and 18 confirmed gHAT cases. Enrolled individuals underwent a dermatological examination, and had blood samples and skin biopsies taken and examined for trypanosomes by molecular and immuno-histological methods. In confirmed cases, dermatological symptoms were significantly more frequent, relative to seronegative controls. T. b. gambiense parasites were present in the blood of all confirmed cases but not in unconfirmed seropositive individuals. However, trypanosomes were detected in the dermis of all unconfirmed seropositive individuals and confirmed cases. After 6 and 20 months of treatment, dermal trypanosome numbers in skin biopsies of confirmed cases progressively reduced. ConclusionsOur results thus highlight the skin as a potential reservoir for trypanosomes, with implications for our understanding of this disease’s epidemiology in the context of its planned elimination and highlighting the skin as a novel target for gHAT diagnostics.

Sadissou Ibrahim、Jamonneau Vincent、Camara Mamadou、MacLeod Annette、Rotureau Brice、Swar Nono-Raymond Kuispond、Cooper Anneli、Camara Oumou、Bart Jean-Mathieu、Bucheton Bruno、Crouzols Aline、Alvarez Estefania Calvo、Travaill¨| Christelle、M?ˉmah Soumah Alseny、Clucas Caroline、Ilbouldo Hamidou、Camara Mariame

Institut de Recherche pour le D¨|veloppement, Unit¨| Mixte de Recherche IRD-CIRAD 177 InterTryp, Campus International de Baillarguet,Institut de Recherche pour le D¨|veloppement, Unit¨| Mixte de Recherche IRD-CIRAD 177 InterTryp, Campus International de Baillarguet,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,Wellcome Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Henry Wellcome Building for Comparative Medical Sciences,Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201 & Department of Parasites and Insect Vectors, Institut Pasteur,Wellcome Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Henry Wellcome Building for Comparative Medical Sciences,||Department of Parasitology, National Institute of Biomedical Research (INRB),Wellcome Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Henry Wellcome Building for Comparative Medical Sciences,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,Institut de Recherche pour le D¨|veloppement, Unit¨| Mixte de Recherche IRD-CIRAD 177 InterTryp, Campus International de Baillarguet,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,||Institut de Recherche pour le D¨|veloppement, Unit¨| Mixte de Recherche IRD-CIRAD 177 InterTryp, Campus International de Baillarguet,Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201 & Department of Parasites and Insect Vectors, Institut Pasteur,Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201 & Department of Parasites and Insect Vectors, Institut Pasteur,Trypanosome Transmission Group, Trypanosome Cell Biology Unit, INSERM U1201 & Department of Parasites and Insect Vectors, Institut Pasteur,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,||Service de Dermatologie, H?pital de Donka,Wellcome Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Henry Wellcome Building for Comparative Medical Sciences,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,||Institut de Recherche en Sciences de la Sant¨| (IRSS) - Unit¨| de Recherche Clinique de Nanoro (URCN),||Institut de Recherche pour le D¨|veloppement, Unit¨| Mixte de Recherche IRD-CIRAD 177 InterTryp, Campus International de Baillarguet,Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Minist¨¨re de la Sant¨|,

10.1101/2020.02.24.20026211

皮肤病学、性病学医学研究方法基础医学

SkinreservoircarriersHuman African TrypanosomiasisTrypanosoma brucei gambiense

Sadissou Ibrahim,Jamonneau Vincent,Camara Mamadou,MacLeod Annette,Rotureau Brice,Swar Nono-Raymond Kuispond,Cooper Anneli,Camara Oumou,Bart Jean-Mathieu,Bucheton Bruno,Crouzols Aline,Alvarez Estefania Calvo,Travaill¨| Christelle,M?ˉmah Soumah Alseny,Clucas Caroline,Ilbouldo Hamidou,Camara Mariame.Dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis[EB/OL].(2025-03-28)[2025-05-17].https://www.medrxiv.org/content/10.1101/2020.02.24.20026211.点此复制

评论