基于“钯标钴染”的基因芯片检测技术初步探索研究

How to develop the new detection method of gene chip with the simple analysis process and no requirement of complicate apparatus in combination with the new labeling of target DNA has been becoming the hot field. And in this paper, we firstly presented the new detection method based on "Palladium Label Cobalt Stain", in which Pd nanoparticles (Pd NPs) were employed to label target DNA instead of the dye molecule and the subsequent cobalt deposition around Pd NPs occurred to amplify the signal through the electroless deposition of cobalt. And the preliminary principal results showed that this technical could efficiently discriminate the target DNA sequence with one base mismatch. In comparison with the "Gold label Silver Stain" presented by Mirkin et al, the above technical can overcome their main shortcoming such as the serious nonspecifically disposition of Silver. Moreover, for the deposition of cobalt, it can be detected not only by the colorimetric but also by the magnetic sensors in future work due to the magnetic property of cobalt. So the above work set the good foundation for the further application of "Palladium Label Cobalt Stain" in the gene chip.