结核分支杆菌Rv2869c拓扑结构的研究

For the further study of the structure of Rv2869c, the topology of Rv2869c was first predicted by web-based topology prediction server. The bacterial alkaline phosphatase (phoA) gene and green fluorescent protein (GFP) gene were then inserted at the periplasmic and cytoplasmic sites of the target protein as the reporter genes respectively. The results showed that Rv2869c harboring four transmembrane helices and the C-terminal located in the periplasm.