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首页|Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT
10.1101/2022.07.22.497959
来源：bioRxiv_logobioRxiv

Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT

Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT

Yelgen Gamze 1Gelsin Beste 2Cavrar Ilayda 2Bal Enes 1Teymur Tarik 3Dogdu Solen 3Araz Hasret 2Erkek Fatmanur 4Sert Berranur 1Ay Yasin 3Yilmaz Menekse Cagla 3Baran Buse 2Tiryaki Nulifer Neslihan 3Tastan Cihan 2Gulden Gamze 1Odabas Sibel Pinar1

1. Molecular Biology, Institute of Science and Technology, ¨1sk¨1dar University||Transgenic Cell Technologies and Epigenetic Application and Research Center (TRGENMER), ¨1sk¨1dar University 2. Transgenic Cell Technologies and Epigenetic Application and Research Center (TRGENMER), ¨1sk¨1dar University||Molecular Biology and Genetics Department, Faculty of Engineering and Natural Science, ¨1sk¨1dar University||HiDNA Biotechnology Inc. 3. Transgenic Cell Technologies and Epigenetic Application and Research Center (TRGENMER), ¨1sk¨1dar University||Molecular Biology and Genetics Department, Faculty of Engineering and Natural Science, ¨1sk¨1dar University 4. Biotechnology, Institute of Science and Technology, ¨1sk¨1dar University||Transgenic Cell Technologies and Epigenetic Application and Research Center (TRGENMER), ¨1sk¨1dar University

生物科学研究方法、生物科学研究技术生物工程学自动化技术、自动化技术设备

Yelgen Gamze,Gelsin Beste,Cavrar Ilayda,Bal Enes,Teymur Tarik,Dogdu Solen,Araz Hasret,Erkek Fatmanur,Sert Berranur,Ay Yasin,Yilmaz Menekse Cagla,Baran Buse,Tiryaki Nulifer Neslihan,Tastan Cihan,Gulden Gamze,Odabas Sibel Pinar.Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT[EB/OL].(2025-03-28)[2025-09-24].https://www.biorxiv.org/content/10.1101/2022.07.22.497959.点此复制

Abstract In manually advancing experimental processes, the stages may be long-term and need to be repeated. Human errors with the repetition of the steps turn into a time-consuming and high-cost for the experiment processes. For this reason, autonomous liquid processing systems are promising technologies. However, in addition to the high cost of fully automatic systems, their maintenance is also quite expensive. Furthermore, conventional systems usually require system-specific protocols and laboratory equipment. Here, we aimed to show that the autonomous robotic systems may provide a closed and error-free molecular biology bench to perform genetic engineering automatically, quickly, and practically 7-24. In this way, researchers can save time from repetitive experiment processes and perform BSL3 experiments including pathogens without human contact. In this study, we built CRISPR.BOT robotic systems to perform Green Fluorescent Protein (GFP) encoding plasmid DNA transfer into bacteria, lentiviral transduction of the gene-of-interests including GFP encoding gene and CRISPR-Cas9 with gRNAs genetic editing system to a human cell line. Furthermore, we showed that CRISPR.BOT system achieved to accomplish single-cell subcloning of GFP+ CRISPR-gRNA+ cells with 90-100% purity. This study suggests that CRISPR.BOT-like approaches may reduce manpower in a safely closed bench in which molecular biology and genetic engineering can be done by robots in a closed system without touching pathogenic microorganisms (virus or bacteria, for example, SARS CoV-2 virus). Furthermore, LEGO Mindstorms robots showed to have the potential to be used in daily laboratory routines with their cost-effectiveness reduced by up to 50 times compared to normal commercial robots.

CRISPRGene EditingRobotic System

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