Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae
Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae
Abstract BackgroundHuman cochlear hair cells cannot regenerate after loss. In contrast, those in fish and amphibians have a remarkable ability to regenerate after damaged. Previous studies focus on mechanisms of hair cell regeneration, such as Wnt and Notch signals. These studies ignore the fact that the beginning of regeneration is accompanied by a large number of inflammatory responses. The role of this inflammation in hair cell regeneration is still unknown. In addition, there is no appropriate behavioral method to quantitatively evaluate the functional recovery of lateral line hair cells after regeneration. ResultsIn this study, we found that when inflammation was suppressed, the regeneration of lateral line hair cells and the recovery of the rheotaxis of the larvae were significantly delayed. Calcium imaging showed that the function of the neuromasts in the inflammation-inhibited group was weaker than that in the non-inflammation-inhibited group at the Early Stage of regeneration, and returned to normal at the Late Stage. Calcium imaging also revealed the cause of the mismatch between the function and quantity during regeneration. ConclusionsOur results, meanwhile, suggest that suppressing inflammation delays hair cell regeneration and functional recovery when hair cells are damaged. This study may provide a new idea for how to promote hair cell regeneration and functional recovery in adult mammals.
Wang Ming、Chen Lin、Li Ya-Juan、Zhang Ru、Liu Xiao-Peng、Hu Bing
CAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China||Auditory Research Laboratory, University of Science and Technology of ChinaCAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China||Auditory Research Laboratory, University of Science and Technology of ChinaLab of Neurodevelopment and Repair, University of Science and Technology of ChinaLab of Neurodevelopment and Repair, University of Science and Technology of China||Auditory Research Laboratory, University of Science and Technology of ChinaAuditory Research Laboratory, University of Science and Technology of China||Center for Hearing and Deafness, State University of New York at BuffaloCAS Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China||Lab of Neurodevelopment and Repair, University of Science and Technology of China
基础医学生理学耳鼻咽喉科学
inflammationhair cell regenerationneuromastlateral linezebrafish larvarheotaxiscalcium imaging
Wang Ming,Chen Lin,Li Ya-Juan,Zhang Ru,Liu Xiao-Peng,Hu Bing.Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae[EB/OL].(2025-03-28)[2025-05-03].https://www.biorxiv.org/content/10.1101/2020.02.26.962753.点此复制
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