肉毒碱脂酰转移酶在高山被孢霉中的沉默

Mortierella alpina is a type of fungus used for the industrial production of polyunsaturated fatty acids. In metabolic engineering, the inhibition of degradation pathway is an important way to increase the accumulation of the product. In this study, transcription levels of most of the β-oxidation-related genes were analyzed, and carnitine acyl-transferase was speculated to be the key enzyme involved in the oxidation. To suppress the oxidation of fatty acids, RNA interference technology was used to achieve gene silence of the key enzyme in M. alpina. Enzyme activity analysis showed the specific activity decreased by 33.3%. Strains were cultivated for 10 days at 28 C with shaking at 200 rpm. The fatty acid accumulation reached up to 38.4% of DCW in transformants, which is higher than the content in wild-type M. alpina. Our research provided theoretical support for implications for industrial production of fatty acids in M. alpina.