抗Sj14-3-3特异性IgY的制备及其用于日本血吸虫病循环抗原的诊断
Preparation of specific yolk immunoglobulin (IgY) of Anti-Sj14-3-3 to serological diagnosis of Schistosomiasis japonica
摘要 目的 制备抗重组日本血吸虫信号转导蛋白14-3-3（Sj14-3-3）的IgY，建立双抗体夹心ELISA诊断体系(IgY-ELISA)，探讨用于日本血吸虫病的诊断价值。方法 利用Sj14-3-3制备并纯化卵黄抗体IgY和兔多克隆IgG抗体；以IgG为捕获抗体，IgY为检测抗体建立双抗体夹心ELISA诊断方法，检测急性血吸虫病患者血清72例，慢性血吸虫病患者128例，正常人血清132例，肺吸虫患者42例，华支睾吸虫患者60例和钩虫感染者血清46例。探讨基于IgY的循环抗原检测的应用价值。结果 成功制备并鉴定了抗Sj14-3-3特异性IgY和IgG抗体，建立了双抗体夹心ELISA检测体系；检测急性血吸虫病患者和慢性血吸虫病患者血清的阳性率分别为73.6 %和43.4 %，正常人血清的阴性率为94.7 %；与肺吸虫患者、华支睾吸虫患者、钩虫感染者血清的交叉反应分别为7.1 %、6.7 %、6.5 %。结论 建立了基于IgY的双抗夹心ELISA检测体系，用于日本血吸虫病患者血清的检测具有较高的敏感性和特异性，可用于日本血吸虫病的辅助诊断。
ITo use yolk immunoglobulin (IgY) instead of IgG antibodies generated from mammalian animals and establish sandwich ELISA to detect the circulating antigens of Schistosoma japonicum for the diagnosis of schistosomiasis. Prepared and purified IgY and IgG antibodies against rSj14-3-3 from the yolk and the sera of rabbits immunized with rSj4-3-3. Double antibody sandwich ELISA using the specific IgG for capture and IgY for detection was set up to test sera of cases, including 72 cases of acute and 128 cases of chronic schistosomiasis. 132 sera of normal individuals were used as control. Additionally, 42 patients with paragonimiasis, 60 with clonorchiasis and 46 with hookworm infections were subjected to cross-reaction investigation. The positive rates of acute and chronics schistosomiasis were 73.6 % and 48.4 % when IgY was used as detection antibody. The specificity of normal human sera was 94.7 %; the cross-reaction with paragonimiasis, clonorchiasis, and hookworm infections were found to be 7.1 %, 6.7 %, and 6.5 %, respectively. IgY antibody used for the detection of circulating antigens has a potential and practical value in the diagnosis of schistosomiasis.
沈继龙、周银娣、罗庆礼
基础医学生物科学研究方法、生物科学研究技术药学
血吸虫病循环抗原14-3-3蛋白卵黄抗体
Schistosomiasis japonicumCirculating antigensignaling protein 14-3-3yolk immunoglobulin (IgY)
沈继龙,周银娣,罗庆礼.抗Sj14-3-3特异性IgY的制备及其用于日本血吸虫病循环抗原的诊断[EB/OL].(2012-03-14)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201203-463.点此复制
评论