尿素循环代谢物富马酸调控巨噬细胞炎症机制的研究

he urea cycle is heterogeneous in the process of macrophage polarization, and different polarization stimulation modes will lead to different metabolic patterns of the urea cycle, and fumaric acid, a metabolite of the urea cycle, has been shown to have an important regulatory effect on macrophages, but the mechanism remains to be determined. In this paper, the production of macrophage pro-inflammatory factors NO, TNF-a, IL-6 and IL-1b was inhibited by ELISA and qPCR, and dimethyl fumarate was succinated and inhibited by silver staining and IP experiments. In summary, this article explains the mechanism of fumarate inhibiting the production of macrophage pro-inflammatory factor NO, and provides new insights into the regulation of macrophage polarization by urea cycle.