Nanozyme-strip for rapid local diagnosis of Ebola
Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. (C) 2015 Elsevier B.V. All rights reserved.
Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. (C) 2015 Elsevier B.V. All rights reserved.
Zhang, Panhe、Gao, George Fu、Qiu, Xiangguo、Liang, Mifang、Liu, Wei、Kobinger, Gary P.、Tan, Shuguang、Gao, George Fu、Fan, Kelong、Zhang, Jianlin、Yan, Xiyun、Duan, Demin、Zhang, Dexi、Liu, Yang
医学研究方法生物科学研究方法、生物科学研究技术预防医学
LATERAL FLOW IMMUNOASSAYMAGNETIC NANOPARTICLESMOLECULAR CHARACTERIZATIONMONOCLONAL-ANTIBODIESNONHUMAN-PRIMATESVIRUS DISEASESSAYBIOSENSORGLYCOPROTEININFLUENZA
Zhang, Panhe,Gao, George Fu,Qiu, Xiangguo,Liang, Mifang,Liu, Wei,Kobinger, Gary P.,Tan, Shuguang,Gao, George Fu,Fan, Kelong,Zhang, Jianlin,Yan, Xiyun,Duan, Demin,Zhang, Dexi,Liu, Yang.Nanozyme-strip for rapid local diagnosis of Ebola[EB/OL].(2016-05-11)[2025-08-02].https://chinaxiv.org/abs/201605.01271.点此复制
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