急性髓系白血病患者骨髓细胞相关分子标志物表达研究
Study of biomarkers expression related with Bone Marrow Cells in patients with Acute Myelogenous Leukemia
目的：探讨急性髓系白血病患者骨髓细胞相关分子标志物（PTEN、mTOR、NF-kB、CD44、PI3K）表达。方法：选取20例住院患者，抽取骨髓液，先涂片检查骨髓白血病细胞百分比，并分临床缓解（9例）与未缓解（11例）两组。再将剩余的骨髓液以石蜡包埋，常规切片，免疫组化技术检测PTEN、mTOR、NF-kB、CD44、PI3K表达。结果：光学显微镜观察缓解组与未缓解组PTEN、mTOR、NF-kB、CD44、PI3K阳性细胞表达率分别为33.3、33.3、77.8、22.2、0与63.6，18.2，90.9，63.6，0。经统计学处理，PTEN、CD44两组比较，有统计学意义（p<0.05）荧光表达强度与图像分析也得出同样的研究结果。结论：急性白血病骨髓细胞PTEN、CD44高表达可作为急性髓系白血病难治诊断和预后判定的重要参考指标。
Objective:To detect the expression of biomarkers（PTEN、mTOR、NF-kB、CD44、PI3K） related with Bone Marrow Cells in patients with Acute Myelogenous Leukemia.Methods:The immunohistochemical method was used to detect the expression of PTEN、mTOR、NF-kB、CD44、PI3K in 20 patients,which were separated into remission group（9 cases）and no-remission group(11 cases) after calculating the percentage of leukemia cells in bone marrow.Results: According to Optical microscope detection,the positive expression rates of PTEN、mTOR、NF-kB、CD44、PI3K in remission group were 33.3%，33.3%，77.8%，22.2%，0.Meanwhile,in no remission group,the positive expression rates were 63.6%，18.2，90.9，63.6%，0.There were statistical differences in the percentage and mean optical density for PTEN 、CD44 between the two groups（p<0.05）.The same as the results detected from Image pro plus 6.0 analysis system.Conclusion:High expression of PTEN、CD44 could be an important index for diagnosis and prognosis in Acute Myelogenous Leukemia.
赖宗浪、李冬云、陈信义、黄晓、张宇、褚雨霆
内科学肿瘤学基础医学
急性髓系白血病骨髓细胞分子标志物
cute Myelogenous LeukemiaBone Marrow CellsSignal Passway biomarkers
赖宗浪,李冬云,陈信义,黄晓,张宇,褚雨霆.急性髓系白血病患者骨髓细胞相关分子标志物表达研究[EB/OL].(2013-11-27)[2025-07-25].http://www.paper.edu.cn/releasepaper/content/201311-564.点此复制
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