多糖分子量与胆固醇连接臂长度对胆固醇改性普鲁兰自组装纳米粒子性质的影响
he influence of Pullulan molecular weight and spacer length on the property of self-assembled nanoparticle formed by cholesterol-modified Pullulan
多糖引入疏水基团后，在水中能自组装形成胶束，可作为疏水小分子药物或生物活性大分子(多肽、蛋白和基因)的载体，在生物医药领域有着广泛的应用价值。本文使用胆固醇为疏水配基，琥珀酸酐和己基二异氰酸酯为连接臂，对分子量为10万和20万的普鲁兰多糖进行疏水改性，制备了2种胆固醇取代度的8种多糖样品，使其进行自组装，利用荧光探针法、动态光散射和透射电镜，考察了多糖分子量与胆固醇连接臂长度对自组装纳米粒子形态、性质的影响规律，为其后期作为药物载体应用奠定了较好的理论基础。
his kind of micelle can be used as hydrophobic drugs or bioactive molecules carriers and has potential applications in biomedical field. In this paper, using cholesterol as hydrophobic ligand, succinic anhydride and 1,6-hexyldiisocyanate as spacers, hydrophobic modified Pullulans with molecular weight about 100,000 and 200,000 were prepared. Self-assembled nanoparticles were then formed in the aqueous solution. The influence of Pullulan molecular weight and spacer length on the property of self-assembled nanoparticle were then investigated by fluorescence study, dynamic laser scattering and transmission electron microscopy.
张其清、张明明、白永刚、杨文智
基础医学生物化学生物工程学
普鲁兰自组装多糖分子量连接臂
Pullulanself-assemblepolyssacharide molecular weightspacer
张其清,张明明,白永刚,杨文智.多糖分子量与胆固醇连接臂长度对胆固醇改性普鲁兰自组装纳米粒子性质的影响[EB/OL].(2012-11-14)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201211-224.点此复制
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