RR大豆深加工制品三重巢式PCR检测技术优化
riplex nested PCR Method for Detecting Roundup Ready Soybean in Highly Processed Products
目前国际上尚未建立有效的大豆深加工产品转基因检测方法。研究旨在建立和优化三重巢式PCR检测技术，并将此技术用于检测RR大豆深加工产品（卵磷脂、大豆蛋白质粉、巧克力饮品、婴儿米粉、大豆粗油、大豆精炼油和大豆色拉油）中多个转基因成分（Lectin、CaMV 35S、CTP、CP4-EPSPS、NOS）。结果显示，三重巢式PCR检测方法适用于大豆深加工产品的定性检测，优于已有的PCR检测方法。
Nowadays, there still have not an effective detection method for the highly processed genetically modified products. A novel method of the triplex nested PCR, was developed for the sensitive detection of several foreign genes insert in RR soybean (Lectin、CaMV 35S、CTP、CP4-EPSPS、NOS) in highly processed products. We detected seven representative highly processed products samples (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, crude soybean oil, soybean refine oil, soybean salad oil) by the triplex nested PCR. The result indicates the advanced level of the method for the genetically modified products detection. It is a flexible assay to detect the RR soybean in highly processed products.
于艳波、高学军、张明辉、敖金霞
遗传学分子生物学生物工程学
深加工产品三重巢式PCR转基因检测
highly processed productstriplex nested PCRGMO detection
于艳波,高学军,张明辉,敖金霞 .RR大豆深加工制品三重巢式PCR检测技术优化[EB/OL].(2006-05-24)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/200605-301.点此复制
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