无机盐对纤维素降解真菌产内切β-D-1,4-葡聚糖酶的影响

he effect of inorganic salts on the production of endo-β-D-1,4-glucanase of cellulose-degrading fungi was investigated in this research. A orthogonal design was applied to design sixteen combinations which included four factors and two level, and the interaction was considered. Visual analysis showed that among the single factor, K2HPO4•3H2O, MgSO4•7H2O and CaCI2 had positive influence on the enzyme production, the absolute value of Ri was respectively 3.885, 0.632 and 0.313, FeSO4•7H2O had negative influence on the enzyme production, the absolute value of Ri was 13.765. Within the interaction, FeSO4•7H2O×MgSO4•7H2O, K2HPO4•3H2O×MgSO4•7H2O, CaCI2×MgSO4•7H2O and CaCI2×K2HPO4 •3H2O had positive impact, the absolute value of Ri was separately 3.841, 3.750, 1.792 and 0.579，while CaCI2×FeSO4•7H2O and FeSO4•7H2O×MgSO4•7H2O had negative impact，the absolute value of Ri was separately 2.472 and 2.070. The optimized combination was as follows, 0.1g CaCI2, 0.1g FeSO4•7H2O, 0.5g K2HPO4•3H2O and 0.5g MgSO4•7H2O in every litre of liquid medium, the enzyme activity was improved 111.916% compared to the control without adding inorganic salts. The result of statistical analysis indicated that the low concentration of FeSO4•7H2O and the high concentration of K2HPO4•3H2O，CaCI2 and MgSO4•7H2O can achieve the maximum of enzyme production.