慢性脑缺血小鼠脑内微血管密度观察

o observe the changes of microvessel density in mice after chronic cerebral hypoperfusion. Methods: (1) 12 KM mice randomized into two groups: ischemic group and control group. The operation of left common carotid artery ligation and transient right common carotid artery ligation was only applied to ischemic group. 2,3,5-triphenyltetrazolium chloride staining was performed to evaluate infarct volume of brain 7 days after brain ischemia. (2) 12 TIE2-GFP transgenic mice randomized into two groups: ischemic group and control group. The same operation was applied to ischemic group. Frozen sections were prepared to count microvessel density with fluorescence microscope at cortex, basal ganglia, and corpus callosum of right and left sides 7 days after brain ischemia. Results: (1) Brain TTC staining: There are 3 mice`s brain slices lightly stained in ischemic group , compared with control group. (2) Microvessel density comparisons of left cortex, left basal ganglia, left corpus callosum, right cortex, right basal ganglia, right corpus callosum between ischemic group and control group show no significant differences (all P>o.o5) . Microvessel density comparisons between right and left sides of cortex, basal ganglia , corpus callosum of ischemic group show no significant differences (all P>o.o5). Conclusion: The microvessel density of cortex, basal ganglia, corpus callosum show no significant changes 7 days after brain ischemia under current experimental circumstances.