蒭雷草对盐胁迫的生理响应
为探讨蒭雷草对逆境的适应能力,揭示其耐盐机理;作者从西沙东岛采集蒭雷草,通过分株繁殖挑选健壮植株为材料;模拟热带珊瑚岛生境设置不同浓度NaCl 处理,研究不同程度的盐胁迫对其植株叶片丙二醛(MDA)、抗氧化酶以及渗透调节物质的影响。结果表明:(1)短期(28 d)盐胁迫下,NaCl 浓度的增加并未加速蒭雷草叶片细胞发生膜脂过氧化作用,MDA 含量增加幅度较小;随着盐胁迫时间延长及NaCl 浓度增加,蒭雷草叶片细胞膜脂过氧化损伤的程度加深,MDA 含量逐渐上升,最大值出现在400 mmol·L-1。(2)短期(28 d)盐胁迫,低浓度(200 mmol·L-1)NaCl 显著提高超氧化物歧化酶(SOD)活性,高浓度(400 mmol·L-1)NaCl 显著提高过氧化氢酶(CAT)活性;而过氧化物酶(POD)活性随盐胁迫时间的延长及浓度的增加逐渐升高。(3)蒭雷草在盐胁迫环境下,机体组织不断积累可溶性蛋白(SP)和脯氨酸(PRO)含量以提高渗透调节能力,平衡细胞内外渗透势,从而达到缓解盐害的目的。整个盐胁迫过程中,蒭雷草机体组织将抗氧化酶防御系统和渗透调节机制相结合,减缓了细胞膜脂过氧化的损伤和细胞失水带来的生理干旱,表现出较强的耐盐能力。研究结果可为蒭雷草在南海诸岛人工群落构建、植被恢复中的应用以及营造良好生态环境提供科学支撑,对中国热带珊瑚岛可持续发展具有重大意义。
In order to test the ability of Thuarea involuta to adapt to adversity and to reveal itsmechanism of salt tolerance, we collected samples from the tropical coral xisha dongdao island,and selected vigorous plants as the subsequent experiment materials through vegetativepropagation, which were then treated with different salt stress degree. We studied the effects oftreatments with different NaCl concentration on the contents of Malondialdehyde (MDA),antioxidase and osmotic regulator in leaves of T. involuta. Our results showed that the increase ofNaCl concentration did not accelerate the membrane lipid peroxidation of T. involuta leaf cellsunder a short term of salt treatment (28 d), but the peroxidation effect worsened with the extensionof salt treatment time and increase of NaCl concentration, which also, resluted in a graduallyrising of the content of MDA, reaching the peak at 400 mmol-1 of NaCl concentration. Under theshort term of salt stress treatment (28 d), the activity of SOD and CAT were significantlyincreased under the low (200 mmol-1) and high (400 mmol-1) NaCl concentration,respectively, the activity of POD gradually ascended with the increase of salt stress time and NaClconcentration. We discovered that under salt treatment, T. involuta accumulated SP and PRO toimprove the osmotic regulation ability, which balanced the osmotic potential inside and outsidethe cell membranes and lowered the demage of salt stress. In conclusion, T. involuta can applyboth of the antioxidant enzyme defense system and osmotic regulation mechanism under saltstress, to reduce the damage caused by lipid peroxidation of cell membrane and to easephysiological drought. In this case, we regarded the species has high salt tolerance. The results canprovide scientific foundation for artificial community construction of T. involuta, vegetationrestoration and landscaping design on South China Sea Islands, and it has great significance forsustainable development to tropical coral islands in China.
李昕、刘东明、赵文忠、陈意兰、李新杰、廖海民
环境生物学植物学生理学
蒭雷草,盐胁迫,丙二醛,抗氧化酶,渗透调节,生理响应
李昕,刘东明,赵文忠,陈意兰,李新杰,廖海民.蒭雷草对盐胁迫的生理响应[EB/OL].(2021-04-29)[2025-08-19].https://chinaxiv.org/abs/202105.00033.点此复制
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