药用动物乌梢蛇线粒体基因组全序列分析
nalysis of Mitochondrial Genome of Ptyas dhumnades (Cantor, 1842)
摘要：【目的】 获取药用动物乌梢蛇线粒体基因组全序列。 【方法】采用Ion Torrent PGM技术，对乌梢蛇全基因组DNA进行提取测序，采用MIRA方法从头组装方法和近缘种mapping方法进行组装拼接，应用MITOS等方法进行注释。 【结果】获得17 162 bp乌梢蛇线粒体基因组全序列。其共编码22个tRNA，13个蛋白编码基因，2个rRNA（16S rRNA、12S rRNA）并有1个轻链（L链）复制起始区。【结论】获取乌梢蛇线粒体基因组全序列，并可用于该药用动物的资源调查与保护研究。
bstract: [Objective] To investigate the complete mitochondrial genome of medical animal Ptyas dhumnades (Cantor, 1842). [Methods] The Ion Torrent PGM technique was chosed to sequence genomic DNA of Ptyas dhumnades, and then the strategy of de novo assembly and mapping versu mtgenome of closely related species by MIRA 4.0 was adopted to get the complete mitochondrial sequence. This genome was annotated by Online server MITOS etc. [Results] The 17 162 bp complete mitochondrial genome of Ptyas dhumnade with high coverage contains 22 transfer RNA, 13 protein coding genes, 2 ribosomal RNA genes and 1 putative L-strand replication origin. [Conclusion] The mtgenome of Ptyas dhumnades was obtained, and could be furher applied in the resources survey and protection of this medical animal.
马光银、齐迎菊、田晓轩、刘杰
生物科学研究方法、生物科学研究技术生物资源调查动物学
线粒体基因组乌梢蛇序列分析
mitochondrial genomePtyas dhumnadessequence analysis
马光银,齐迎菊,田晓轩,刘杰.药用动物乌梢蛇线粒体基因组全序列分析[EB/OL].(2016-05-31)[2025-07-16].http://www.paper.edu.cn/releasepaper/content/201605-1599.点此复制
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