环境因子对黄姑鱼精子活力的影响
Effects of Environmental Factors on the Vitality of Sperm in Nibea albiflora
通过测定黄姑鱼精子的激活率、运动时间及寿命研究了不同环境因子对黄姑鱼精子活力的影响。结果表明,黄姑鱼精液中精子浓度为(1.41±0.12)×1010个/ ml;精子激活与运动的适宜盐度为25~35、适宜pH为7.5~8.5。在pH 8.0~8.3、盐度25条件下,精子激活率达(87.00±5.54)%,运动时间及寿命分别为(336±14.02)s及(405.33±12.22)s。精子激活与运动的适宜NaCl、KCl、MgCl2及葡萄糖溶液浓度分别为300~500mmol•L-1、600mmol•L-1、800~1000 mmol•L-1及900 mmol•L-1;精子在CaCl2溶液中有聚集现象出现,且运动时间及寿命明显缩短;精子在缺少HCO3-的人工海水中未能被激活;精子在无Ca2+或Mg2+的人工海水中激活率均超过80%,但运动时间及寿命均有所缩短。
he effects of environmental factors on the sperm vitality of Nibea albiflora were investigated. The results indicated that the density of sperm was (1.41±0.12) ×1010 / ml; the suitable salinity for sperm activation and movement was 25~35 while pH value 7.5~8.5. The highest activation rate, the longest moving time and life-ban of sperm were(87.00±5.54)%,(336±14.02)s、(405.33±12.22)s, respectively when the sperm was activated in the solutions of salinity 25. And the suitable oncentration of KCl, NaCl, MgCl2, glucose solutions for sperm activation were 300~500mmol•L-1, 600mmol•L-1, 800~1000 mmol•L-1, 900 mmol•L-1. But the sperm had some conglomeration and moving time shorten quickly in the CaCl2 solutions. the sperm could not be activated by HCO3¯free artificial seawater. More than 80% of sperms were activated by Ca2+-free, Mg2+- free artificial seawater, but swam lasted short time.
魏平、闫家强
环境生物学动物学生理学
黄姑鱼精子活力环境因子
Nibea albifloraspermvitalityenvironmental factors
魏平,闫家强.环境因子对黄姑鱼精子活力的影响[EB/OL].(2009-07-02)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/200907-53.点此复制
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