大豆花叶病毒病抗源东农93046对N3株系成株及种粒斑驳抗性位点分析

Soybean mosaic virus (SMV) is a prevalent virus，which seriously impacts on soybean yield and quality. Based on previous study, one recombinant intercross line (RIL) population from a cross between Donong93046(with resistance to plant and seed coat mottle of SMV N3) and Pin1246(with susceptible to plant and seed coat mottle of SMV N3) were used to analyze plant and seed coat mottle resistance inheritance mode of Donong93046, to map SMV resistant gene loci through SSR markers, and to obatain SSR markers assoiated with resistant gene loci for marker assistanat breeding. The result of the inheritance mechanism of plant and seed coat mottle resistance in Donong93046 was both quality trait, which were both contolled by a single resistant gene. A molecular genetic map of F linkage group was consructed based on 13 original SSR markers and 22 new developed SSR markers, which covered 260.1cM (with mean marker distance:7.44 cM). The plant and seed coat mottle resistance gene loci were both associated with SSR marker Satt114, Satt334 and Satt362, which were located in similar genomic regions. Furthermore, the coincidence ratio of associated SSR marker in phenotype and genotye of line were very high, which could be used to marker assistant breeding.