淡色库蚊抗药性piRNA测序

he mosquito-borne diseases are important infectious diseases in the world and caused serious threat to human health. Chemical control, because of its killing speed, long-lasting and convenient features, has been the main method in the comprehensive management strategy. Unfortunately, unreasonable and continuous usage of insecticides induced the development and spread of resistance. Then insecticide resistance exacerbated the spread of mosquito-borne diseases. However, after a very long time, chemical control for its rapid and efficient advantage will still be the main method in control of mosquito-borne diseases.Insecticide resistance mechanisms include three main areas: target resistance, that is, the change of the insecticide target sites. Metabolic resistance, that is, the change of metabolic enzymes transcription level involved in metabolic resistance, and physiological resistance and behavior avoidance also involved in pestcide resistance, such as cuticle protein and chitin enzyme also involved in insecticide resistance.Piwi-interacting RNAs ( piRNAs ), 26-31nt, are a new class small non-coding RNA (sncRNA) found in 2006 in several laboratory.In this research,piRNA expression profiling of pestcide resistence in Culex pipiens pallensmosequito.112608 piRNA unique clean reads were gotten using Illumina Hiseq 2000 technology, and 50445（44.8%）clean reads mapping to Cx. pipiens quinquefasciatus.The unique piRNA typle number of DS and DR strain is 40456 and 15519 respectively. We got 515198 piRNA reads in DS and the number in DR is 222160. Meanwhile, the aligned piRNA number in repetitive sequence of DS, DR and pooled sample are 20343, 10889 and 16090 respectively. Matched piRNA show no tendency in DS,DR and mixed sample. PiRNAs from the genome show the similar result. piRNAs widespread express in genes, no matter where they are. Those mapping piRNA show two peaks in 28nt and 31nt in length.The differently expressed piRNAs is50445, the number high express in DS, DR is 37608,12837 respectively.