m 6 A RNA methylation impairs gene expression variability and reproductive thermotolerance in Arabidopsis
m 6 A RNA methylation impairs gene expression variability and reproductive thermotolerance in Arabidopsis
Abstract Plants are more susceptible to high temperature stress during reproductive development, which can cause drastic yield loss of fruit and seed crops. Unfortunately, the underlying mechanism remains largely unknown. Here we suggest that m6A RNA methylation level increases in the reproductive tissues of Arabidopsis and negatively regulates gene expression variability. It has been suggested that stochasticity of gene expression can be advantageous to fitness of living organisms under environmental challenges. Indeed, reduced gene expression variability in flowers was associated with compromised transcriptional activation of heat-responsive genes. Importantly, disruption of an RNA demethylase AtALKBH10B led to lower gene expression variability, hypo-responsiveness of heat-activated genes, and strong reduction of plant fertility. Overall, our work proposes a novel mechanism that m6A RNA modification mediates the bet-hedging strategy of plants challenged by heat stress.
Zhuang Haiyan、Fan Wenwen、Wang Ling、Yang Hongxing、Zhang Xia、Cho Jungnam、Dong Haihong
Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden||National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences||University of Chinese Academy of ScienceShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden||National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences||University of Chinese Academy of ScienceShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical GardenShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden||National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences||University of Chinese Academy of Science||CAS-JIC Centre for Excellence in Plant and Microbial ScienceShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden
植物学遗传学分子生物学
m6A RNA methylationgene expression variabilityheat toleranceAtALKBH10BArabidopsis thaliana
Zhuang Haiyan,Fan Wenwen,Wang Ling,Yang Hongxing,Zhang Xia,Cho Jungnam,Dong Haihong.m 6 A RNA methylation impairs gene expression variability and reproductive thermotolerance in Arabidopsis[EB/OL].(2025-03-28)[2025-05-26].https://www.biorxiv.org/content/10.1101/2022.03.25.485737.点此复制
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