迟缓爱德华氏菌对鱼血浆反应的差异蛋白质组学研究
ifferential proteomic approach for Edwardsiella tarda in response to fish plasma
虽然已有文献报道迟缓爱德华氏菌具有抵抗血清杀菌作用，但其分子机制尚不清楚。为此，将同等菌量的迟缓爱德华氏菌与不同体积的鱼血浆孵育，获取全菌蛋白，进行基于等电聚焦双向电泳的蛋白质组学分析，发现YihK和GpmA为显著上调的差异蛋白。这些发现有助于阐明病原菌对鱼血浆反应的分子机制。
Previous reports indicated that Edwardsiella tarda showed serum assistance, but information regarding to its molecular mechanism is not available. In the present study, equal amount bacteria were incubated with different volumes of fish plasma and then bacterial proteins were extracted for 2-DE-based proteomics. Upregulation of YihK and GpmA were detected. The finding is helpful in clarification of molecular mechanism of E. tarda in response to fish plasma.
许文姣、李惠
微生物学生物化学分子生物学
迟缓爱德华氏菌蛋白质组学血浆
Edwardsiella tardaproteomicsplasma
许文姣,李惠.迟缓爱德华氏菌对鱼血浆反应的差异蛋白质组学研究[EB/OL].(2010-06-18)[2025-04-27].http://www.paper.edu.cn/releasepaper/content/201006-364.点此复制
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