荭草花化学成分指纹图谱研究
Study on fingerprints of chemical constituents of Flowers Polygoni Orientalis
目的： 建立荭草花HPLC特征指纹图谱，为该药材的品质评价提供参考。方法：以花旗松素为参照物，采用HPLC法，Diamonsil C18（4.6 mm×200 mm，5 μm）色谱柱；流动相为乙腈-0.1%磷酸水（梯度洗脱）；检测波长为280 nm；流速为1.0 mLomin-1；柱温为30 ℃。结果：12个不同产地的荭草花药材指纹图谱标定出18个共有峰，其中指认出4个共有峰，分别为花旗松素，儿茶素，没食子酸，3,3'-二甲氧基鞣花酸-4-O-β-D-葡萄糖苷。通过"中药色谱指纹图谱相似度评价系统"软件计算，12个不同产地荭草花指纹图谱相似度均＞0.9。结论：本实验方法重现性好，指纹图谱专属性强，可为荭草花药材的鉴别及质量控制提供科学依据。
Objective:To establish the HPLC fingerprint method of Flowers Polygoni Orientalis. Methods:HPLC, Diamonsil C18 column(4.6 mm × 200 mm, 5 μm), mobile phase was acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength was 280 nm and flow rate was 1.0 mLomin-1,the column temperature was 30 ℃. Results: Eighteen common peaks were pointed out from the HPLC fingerprint of 9 places of Flowers Polygoni Orientalis; Flowers Polygoni Orientalis different batches were of strong similarity.Conclusion: The repetition，stability and precision of the method is good and it can be used to control the quality of Flowers Polygoni Orientalis.
佟苗苗、康晓燕、翟延君、张宇瑶、初正云
药学
荭草花HPLC指纹图谱花旗松素等共有峰
Flowers Polygoni OrientalisHPLCFingerprint Analysiscommon peaks of taxifolinetc
佟苗苗,康晓燕,翟延君,张宇瑶,初正云.荭草花化学成分指纹图谱研究[EB/OL].(2013-05-02)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201305-26.点此复制
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