浆果类果树EST-SSR标记的开发及应用

With the development of expressed sequence tags (ESTs) data, expressed sequence tag-simple sequence repeat markers (EST-SSR) had been utilized most extensively as a new marker assistant breeding method for plant genetic research, which not only due to their advantages over SSR markers, also possessed merits as stability, low cost-efficient for development, high polymorphism and high transferability. In a sense, functional information might be obtained directly or indirectly, since EST-SSRs were found in expressed gene, in that case the germplasm resources of berry fruits with significantly agronomic trait could be explored. Thus, the EST-SSR studies of berry fruits in the last few years which including construction of genetic map, genetic diversity, relationship identification and species transferability were reviewed. At this point, by the use of EST-SSR markers the working processes of exploring new gene, breeding new varieties as well as identifying genetic relationship would be enhanced, and then promote the breeding process across berry fruits.