组蛋白去甲基化酶KDM7A促进HBV的复制
Histone demethylase KDM7A promotes the replication of HBV
目的：检测组蛋白去甲基化酶KDM7A对HBV复制的影响。方法：利用实时荧光定量PCR、Western Blot、ELISA检测KDM7A对HBV复制的影响；利用双荧光素酶报告基因检测KDM7A对HBV相关启动子的影响。结果：HBV可以促进KDM7A mRNA的转录及蛋白表达；KDM7A可促进HBV基因组的复制及病毒抗原的产生，增强HBV相关启动子的激活。结论：组蛋白去甲基化酶KDM7A促进HBV的复制。
OBJECTIVE: To detect the effect of histone demethylase KDM7A on HBV replication. METHODS: The effects of KDM7A on HBV replication were detected by real-time fluorescence quantitative PCR, Western Blot and ELISA; the effects of KDM7A on HBV-related promoters were detected using dual luciferase reporter genes. RESULTS: HBV can promote the transcription and protein expression of KDM7A; KDM7A can promote the genome replication and viral antigen production of HBV and enhance the activation of HBV-related promoters. CONCLUSION: Histone demethylase KDM7A promotes the replication of HBV.
朱海珍、杨迪、田仁云
基础医学分子生物学生物化学
病毒学HBVKDM7A
VirologyHBVKDM7A
朱海珍,杨迪,田仁云.组蛋白去甲基化酶KDM7A促进HBV的复制[EB/OL].(2023-03-28)[2025-05-13].http://www.paper.edu.cn/releasepaper/content/202303-328.点此复制
评论