连续切片小鼠窦房结NF-160及起搏通道HCN4表达

Objective: To study the morphological structure and NF-160 and HCN4 expression of sinoatrial node in adult mouse. Method: After being routinely embedded in paraffin, completer serial sections of right and left atria were cut, 10 samples were stained with Masson trichrome and NF-160 immunohistochemistry staining, and observed under light microscope. Ather 5 samples were added NF-160 and HCN4 antibodies by immunofluorescence staining, and observed under confocal microscope. Results: The mouse sinoatrial node was located between the superior vena cava and the auricular dextra. The shape of the mouse sinoatrial node was almost ovoid or irregular in horizontal section. The sinus node artery can be seen clearly through the parenchyma of the sinoatrial node in 8 samples, so the artery can be used as a location mark. NF-160 and HCN4 expressed in pacemaker cells and transitional cells. Conclusion: The mouse sinoatrial node located at a high position. The sinus node artery passed through the parenchyma. The cells express both NF-160 and HCN4.?????