高糖诱导山羊骨骼肌卫星细胞成脂分化过程中相关基因表达的变化

In this paper, the method and mechanism for skeletal muscle satellite cells (SMSCs) adipogenic differentiation were detected in vitro. (Method) High concentration glucose (25 mM/L) culture medium was used to induce the SMSCs. Cells were stained with Oil Red O and qPCR was employed to detect the relative expressions of ACC, FAS, C/EBPα, PPARγ, SREBP-1 and AdipoQ at six time points (0 d, 2 d, 4 d, 6 d, 8 d and 10 d). (Result) The results showed that lots of lipid droplets were observed in cells stained with Oil Red O at the 6 d. The mRNA expressions of ACC and C/EBPα significantly increased at the 2 d (P<0.05) and maintained relative high expression levels at later stages, and the expressions of SREBP-1 and FAS were both significantly up-regulated at the 6 d (P<0.05), while the expression of PPARγ significantly increased at the 10 d (P<0.05). The expression of AdipoQ significantly ascended at the 2 days and 10 d (P<0.05). (Conclusion) In this study, we found that high concentration glucose promoted the expressions of these adipogenic-related genes and induced the SMSCs to differentiate into adipocyte-like cells.