酿酒葡萄中与原花色素生物合成相关的MYB家族转录因子启动子的克隆与功能研究

Proanthocyanidins are an important group of polyphenolic metabolites in grape berries, which have great influences on the taste of grape and wine. In recent years, some regulatory genes related to proanthocyanidin biosynthesis in grape berries were found, and their physical functions were also clarified. Especially the studies of a series of regulator of MYB family in grapes have greatly developed the researches in this area. However, until now the researches for the structures and functions of the promoters of these regulatory genes are still quite limited. In the present study, the promoter sequences of the regulatory genes VvMYBPA1、VvMYBPA2 and VvMYB5a which are related to proanthocyanidin biosynthesis were cloned, their potential functions were predicted with the help of bioinformatics, and their basic functions were tested by using infecting of promoter combinant vector on tobacco leaves. This research revealed that compared to the structure genes related to proanthocyanidin biosynthesis, the promoters of these regulatory genes were quite different in the structure and the protentional functions, especially in the kinds and numbers of the photoresponse elements, and they often contain the response sites to other different regulators or phytohormone signals. Besides, the function test revealed that all these aimed sequences had the basic functions of promoters, and after the irraidation of UV-B the expression of the report genes increased slightly, showing their function in responding the induction of UV.