一种新型独脚金内酯受体激动剂的筛选与功能验证

Strigolactone(SL) is a new kind of plant hormone, which plays a key role in plant type constitution. Exogenous addition of SL and its analogues (such as GR24) plays an important role in agricultural production. However, there are still some problems in the synthesis of SLS analogues, such as difficult synthesis, poor stability and high cost. Based on the three-dimensional structure of the complex of rice SL receptor OSD14 and synthetic analogue GR24, a new SL analoguegx-series was constructed by replacing the unstable enol ether bond with a stable aromatic bond. Then, the biological activities of gx-series compounds were evaluated by hypocotyl inhibition test and yeast two-hybrid test, the results showed that gx01, gx03 and gx04 could inhibit hypocotyl elongation, inhibit rosette leaf branching and promote root hair elongation, and the stability of these compounds was evaluated by HPLC. The results showed that the gx-series compounds were better than GR24 in activity and stability. The results of this paper provide important theoretical guidance for the further development of new and efficient SL analogues.