Nicotine dosimetry and stability in Cambridge Filter PADs (CFPs) following different smoking regimen protocols and condition storage
Nicotine dosimetry and stability in Cambridge Filter PADs (CFPs) following different smoking regimen protocols and condition storage
ABSTRACT Despite the growing numbers of studies with cigarettes and other electronic nicotine delivery products (ENDs), there is no standard covering nicotine dosimetry and its stability in various matrix. The aim of the present study was to provide a protocol to normalize nicotine concentration adsorbed in Cambridge Filter PADs (CFPs) and their storage method. Smoke/vapor generated by a reference tobacco cigarette (1R6F) and ENDs with different exposure regimes (ISO, HCI and CRM81) was collected in CFPs. For each exposure, some CFPs were analyzed at time zero, whereas the others were stored under different conditions for nicotine assessment after 30 days. Principal Component Analysis (PCA) was also performed to establish the best parameter for nicotine normalization. PCA showed the best correlation between nicotine in CFPs and TPM. Our results showed differences between products and puffing regimes, but storage of CFPs at ?80°C was always effective in maintaining the nicotine content. In conclusion, this study highlights that different exposure regimens and products can affect the preservation of nicotine titer in CFPs and samples storage at ?80°C may prevent the loss of nicotine. These conditions are recommended and should be adopted for Inter-laboratory comparison studies on ENDs to ensure harmonization between participating laboratories.
Emma Rosalia、Rust Sonja、Caruso Massimo、Pulvirenti Roberta、Favara Claudia、Zuccarello Pietro、Polosa Riccardo、Ferrante Margherita、Volti Giovanni Li
Department of Biomedical and Biotechnological SciencesCenter of Excellence for the Acceleration of Harm Reduction (CoEAHR), University of CataniaCenter of Excellence for the Acceleration of Harm Reduction (CoEAHR), University of Catania||Department of Biomedical and Biotechnological SciencesDepartment of Biomedical and Biotechnological SciencesDepartment of Medical, Surgical Sciences and Advanced Technologies ?°G.F. Ingrassia?±Department of Medical, Surgical Sciences and Advanced Technologies ?°G.F. Ingrassia?±Center of Excellence for the Acceleration of Harm Reduction (CoEAHR), University of Catania||Department of Clinical and Experimental Medicine, University of CataniaDepartment of Medical, Surgical Sciences and Advanced Technologies ?°G.F. Ingrassia?±||Center of Excellence for the Acceleration of Harm Reduction (CoEAHR), University of CataniaCenter of Excellence for the Acceleration of Harm Reduction (CoEAHR), University of Catania||Department of Biomedical and Biotechnological Sciences
医学研究方法药学生物科学研究方法、生物科学研究技术
nicotineelectronic cigarettestabilityCambridge Filter Padsmoking regimenstorage
Emma Rosalia,Rust Sonja,Caruso Massimo,Pulvirenti Roberta,Favara Claudia,Zuccarello Pietro,Polosa Riccardo,Ferrante Margherita,Volti Giovanni Li.Nicotine dosimetry and stability in Cambridge Filter PADs (CFPs) following different smoking regimen protocols and condition storage[EB/OL].(2025-03-28)[2025-05-08].https://www.biorxiv.org/content/10.1101/2020.09.09.289371.点此复制
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