淋巴细胞乙肝病毒感染状态与临床意义的研究

IObjective Using cells cultured in vitro amplification technology to detect hepatitis B virus DNA load in peripheral blood T lymphocytes and explore the clinical significance.Method: Three cases of hepatitis B virus carriers, 32 cases of chronic hepatitis B patients were involved. Peripheral blood mononuclear cells were separated by density gradient centrifugation over Ficoll-Hypaque, And T cells were purified from the mononuclear cells fraction using human CD3 positive selection kit, The T cell were inoculated into 9/24-well plates, then the cells were cultured for 12 days. Intracellular HBV were examined by immunohistochemistry and situ hybridization, intracellular and supernatant HBV DNA levels were examined at day 0, 1, 6 and 12 .Results: HBsAg that T cells expressed were detected by using immunohistochemistry and a part of HBV-DNA could be seen in T cell by situ hybridization. HBV-mRNA could be detected by RT-PCR, ,and HBV DNA in the supernatant and intracellular increased with the proliferation of T cell. Conclution: HBV could infect and replicate in T cells. The hepatitis B virus can increase with amplification of the T-cell replication. Serum virus detection with T-cell virus detection were positively correlated T-cell virus detection and liver biopsy inflammatory and fibrosis level was positively correlated.