Centrally expressed Cav3.2 T-type calcium channel is critical for the initiation and maintenance of neuropathic pain
Centrally expressed Cav3.2 T-type calcium channel is critical for the initiation and maintenance of neuropathic pain
Abstract Cav3.2 T-type calcium channel is a major molecular actor of neuropathic pain in peripheral sensory neurons, but its involvement at the supra-spinal level is almost unknown. In the Anterior Pretectum (APT), a hub of connectivity of the somatosensory system involved in pain perception, we show that Cav3.2 channels are expressed in a sub-population of GABAergic neurons co-expressing parvalbumin (PV). In these PV-expressing neurons, Cav3.2 channels contribute to a high frequency bursting activity, which is increased in the spared nerve injury model of neuropathy. Specific deletion of Cav3.2 channels in APT neurons reduced both the initiation and maintenance of mechanical and cold allodynia. These data are a direct demonstration that centrally expressed Cav3.2 channels also play a fundamental role in pain pathophysiology.
Fayad Sophie L.、Mallet Christophe、Bourinet Emmanuel、Bessaih Thomas、Le Gac Benjamin、Fruqui¨¨re Antoine、Laffray Sophie、Gasmi Laila、Leresche Nathalie、Jouffre Baptiste、Lamoine Sylvain、Ourties Guillaume、Cauli Bruno、Lambert R¨|gis C.
Sorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Universit¨| Clermont Auvergne, Inserm, U1107 Neuro-Dol, Pharmacologie Fondamentale et Clinique de la Douleur||ANALGESIA Institute, Faculty of MedicineInstitut de G¨|nomique Fonctionnelle, Universit¨| de Montpellier, CNRS, INSERMSorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Sorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Institut de G¨|nomique Fonctionnelle, Universit¨| de Montpellier, CNRS, INSERMInstitut de G¨|nomique Fonctionnelle, Universit¨| de Montpellier, CNRS, INSERMSorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Sorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Universit¨| Clermont Auvergne, Inserm, U1107 Neuro-Dol, Pharmacologie Fondamentale et Clinique de la Douleur||ANALGESIA Institute, Faculty of MedicineUniversit¨| Clermont Auvergne, Inserm, U1107 Neuro-Dol, Pharmacologie Fondamentale et Clinique de la Douleur||ANALGESIA Institute, Faculty of MedicineUniversit¨| Clermont Auvergne, Inserm, U1107 Neuro-Dol, Pharmacologie Fondamentale et Clinique de la Douleur||ANALGESIA Institute, Faculty of MedicineSorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)Sorbonne Universit¨|, CNRS, INSERM, Neurosciences Paris Seine ¨C Institut de Biologie Paris Seine (NPS-IBPS)
神经病学、精神病学基础医学生理学
Anterior PretectumGABAergic neuronsParvalbuminBurstVoltage-dependent calcium channelsSpared nerve injury model
Fayad Sophie L.,Mallet Christophe,Bourinet Emmanuel,Bessaih Thomas,Le Gac Benjamin,Fruqui¨¨re Antoine,Laffray Sophie,Gasmi Laila,Leresche Nathalie,Jouffre Baptiste,Lamoine Sylvain,Ourties Guillaume,Cauli Bruno,Lambert R¨|gis C..Centrally expressed Cav3.2 T-type calcium channel is critical for the initiation and maintenance of neuropathic pain[EB/OL].(2025-03-28)[2025-05-03].https://www.biorxiv.org/content/10.1101/2022.04.27.489708.点此复制
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