实时荧光定量PCR法检测环境假单胞菌属
etection of Abundance of Pseudomonas Sp in Environmental Samples by Real-Time Quantitative PCR
应用实时荧光定量PCR法，建立了总细菌及假单胞菌属的标准曲线，并以环境样本中假单胞菌属与总细菌的Ct比值反映其细菌丰度。应用该方法评价人工除藻反应器中组合填料（RZ）、无纺布（RW）、弹性填料（RT）等介质对太湖水中溶藻细菌之一的假单胞菌属的富集情况。结果显示，本方法对总细菌的检测范围为103～108个基因拷贝/µL (R2=0.997)，假单胞菌属的检测范围为1～105个基因拷贝/µL (R2=0.994)。对除藻反应器溶藻细菌富集效果的评价显示，所建立的方法能有效检测反应器中填料对该类溶藻细菌的富集程度。初步证明所建立的方法可有效定量假单胞菌属在环境样本中的丰度。
real-time quantitative PCR (RTQ-PCR) method was established to quantitatively detect abundance of algicidal bacteria of Pseudomonas Sp in environmental samples in this study. The standard abundance curves of total bacteria and Pseudomonas Sp were established, respectively and the abundance of Pseudomonas sp was demonstrated by the Ct ratio of Pseudomonas Sp and total bacteria. The abundance of algicidal bacteria of Pseudomonas Sp on the artificial mediums (RZ, RW, RT) of algae treatment reactor in Taihu Lake were then detected by the method. The results showed that the determination ranges of total bacteria and Pseudomonas Sp were 103～108 gene copies/µL (R2=0.997) and 1～105 gene copies/µL (R2=0.994), respectively. The accumulation of Pseudomonas sp on the artificial mediums in reactor was found effectively by the assay. It is believed that RTQ-PCR method can detect the abundance of algicidal bacteria of Pseudomonas sp in environmental samples quantitatively.
梁戈玉、尹立红、赵传鹏、李先宁、浦跃朴、吕锡武
环境科学理论微生物学环境生物学
实时荧光定量PCR假单胞菌属人工介质溶藻细菌
real-time quantitative PCRPseudomonas spartificial mediumsalgicidal bacteria
梁戈玉,尹立红,赵传鹏,李先宁,浦跃朴,吕锡武.实时荧光定量PCR法检测环境假单胞菌属[EB/OL].(2005-11-07)[2025-07-16].http://www.paper.edu.cn/releasepaper/content/200511-101.点此复制
评论