氟对体外培养人牙乳头间充质细胞合成I型胶原的影响

Objective To investigate the effects of fluoride on type I collagen in cultured human dental palilla cells. Methods Cultured human dental papilla cells were treated with fluoride of 0.0g/L、0.2×10-6g/L、1.0×10-6g/L、5.0×10-6g/L、25×10-6g/L. Immunocytochemistry and image analysis were introduced to access the content of type I collagen in cells. Results it was showed that type I collagen was stained in human dental palilla cells , however, statistic analysis exhibited that experimental fluoride (0.0g/L、0.2×10-6g/L、1.0×10-6g/L、5.0×10-6g/L and 25×10-6 g/L) had not effects on collagen protein synthesizing in cultured cells. Conclusion collagen I protein synthesizing may not sensitive to fluoride.