Dissecting the localization of Tilapia tilapinevirus in the brain of the experimentally infected Nile tilapia ( Oreochromis niloticus )
Dissecting the localization of Tilapia tilapinevirus in the brain of the experimentally infected Nile tilapia ( Oreochromis niloticus )
Abstract Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging virus that inflicts significant mortality on farmed tilapia globally. Previous studies reported detection of the virus in multiple organs of the infected fish; however, little is known about the in-depth localization of the virus in the central nervous system. Herein, we determined the distribution of TiLV in the entire brain of experimentally infected Nile tilapia. In situ hybridization (ISH) using TiLV-specific probes revealed that the virus was broadly distributed throughout the brain. The strongest positive signals were dominantly detected in the forebrain (responsible for learning, appetitive behavior, and attention) and the hindbrain (involved in controlling locomotion and basal physiology). The permissive cell zones for viral infection were observed mostly to be along the blood vessels and the ventricles. This indicates that the virus may productively enter into the brain through the circulatory system and widen broad regions, possibly through the cerebrospinal fluid along the ventricles, and subsequently induce the brain dysfunction. Understanding the pattern of viral localization in the brain may help elucidate the neurological disorders of the diseased fish. This study revealed the distribution of TiLV in the whole infected brain, providing new insights into fish-virus interactions and neuropathogenesis.
Kayansamruaj Pattanapon、Sangpo Pattiya、Senapin Saengchan、Rung-ruangkijkrai Tilladit、Dinh-Hung Nguyen、Dong Ha Thanh、Rodkhum Channarong、Kruangkum Thanapong
Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart UniversityFaculty of Science and Technology, Suan Sunandha Rajabhat University||Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol UniversityCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University||National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Department of Veterinary Anatomy, Faculty of Veterinary Science, Chulalongkorn UniversityFish Infectious Diseases Research Unit (FID RU), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University||The International Graduate Course of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn UniversityFaculty of Science and Technology, Suan Sunandha Rajabhat UniversityFish Infectious Diseases Research Unit (FID RU), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University||The International Graduate Course of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn UniversityCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University||Department of Anatomy, Faculty of Science, Mahidol University
水产、渔业生物科学研究方法、生物科学研究技术动物学
Tilapia lake virusin situ hybridizationbrainneuropathogenesislocalizationventriclescerebrospinal fluid
Kayansamruaj Pattanapon,Sangpo Pattiya,Senapin Saengchan,Rung-ruangkijkrai Tilladit,Dinh-Hung Nguyen,Dong Ha Thanh,Rodkhum Channarong,Kruangkum Thanapong.Dissecting the localization of Tilapia tilapinevirus in the brain of the experimentally infected Nile tilapia ( Oreochromis niloticus )[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.01.19.427199.点此复制
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