电针对胰岛素抵抗大鼠下丘脑PI3K蛋白表达的影响
Effect of electro-acupuncture on expression of PI3K in insulin resistance model rat
目的：观察"双固一通"电针法对胰岛素抵抗模型大鼠下丘脑中的PI3K及p-PI3K蛋白表达影响。方法：以高脂饮食诱导的诱导胰岛素抵抗模型大鼠。正常组（Group 1）大鼠不给予任何处理，常规饲养;预防组（Group 3）、"双固一通"电针组（Group 4）、脑室灌注脑脊液组（Group 5），和脑室灌注阻滞剂组（Group 6）分别采用"双固一通"法电针治疗。实验开始后的第8w和第16w检测各组大鼠体重(BW)、空腹血糖（FPG）、血浆胰岛素(Fins)水平，及胰岛素抵抗指数（ISI）；West-blot 法检测大鼠下丘脑PI3Kp110及p- PI3Kp110蛋白表达。结果： (1) Group 3 与Group 4大鼠BW、FPG、FINS降低（p<0.05）,IAI升高（p<0.05）； (2) Group 3、 Group 4、大鼠下丘脑中 PI3Kp110蛋白及p- PI3Kp110蛋白表达明显提高（P＜0.05）。结论:"双固一通"电针法可通过调节大鼠下丘脑中 PI3Kp110蛋白及p- PI3Kp110蛋白表达，从而改善胰岛素抵抗模型大鼠的体重、空腹血糖、血浆胰岛素水平，胰岛素敏感性。
Objective: To observe the effects of Double-reinforcing and One-unblocking of acupuncture method on on expression of PI3K and p-PI3K insulin resistance model rat. Methods: Use the high-fat diet induced insulin resistance model of rats. Group 1 rats were feed normal food, Group 3, Group 4, Group 5, Group 6 were feed with high-fat diet daily, and treated with Double-reinforcing and One-unblocking of acupuncture stimulation. BW, FPG, Fins, and IAI were measureed in 8th week and the 16th week respectively; the hypothalamus PI3K expression and p-PI3K expression were detected by western-blot.Result: (1) BW, FPG, Fins were decreased（p<0.05）, and IAI increased（p<0.05） in Group 3 and Group 4; (2) the hypothalamus PI3K expression and p-PI3K expression were increased in Group 3 and Group 4（P＜0.05）. Conclusion：The above results suggest that the effects of Double-reinforcing and One-unblocking of acupuncture method can improve the BW, FPG, Fins, and IAI，probable through regulate the hypothalamus PI3K expression and p-PI3K expression in insulin resistance model rat.
周焕娇、刘建民、梁凤霞、王华、杨丰、李念、唐宏图、王静芝
中医学基础医学内科学
胰岛素抵抗“双固一通”电针下丘脑PI3Kp110p- PI3Kp110蛋白表达
Insulin ResistanceDouble-reinf Double-reinforcing and One-unblocking acupuncture methodHypothalamusPI3K expressionp-PI3K expression
周焕娇,刘建民,梁凤霞,王华,杨丰,李念,唐宏图,王静芝.电针对胰岛素抵抗大鼠下丘脑PI3K蛋白表达的影响[EB/OL].(2013-12-06)[2025-06-29].http://www.paper.edu.cn/releasepaper/content/201312-148.点此复制
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