甜瓜花器官发育相关基因的电子克隆及表达分析

Using transcriptom sequencing technology to analysis and detected different expressed gene among monoecious，andromonecious，gynoecious and hermaphrodite families from WI998 （gynoecious）cross Topmark（andromonecious），a full length of 1 842 bp which locates in chromosome12，named CmTs2，was obtained from bioinformatics methods. Sequence analysis indicated that the CmTs2consisting an open reading frame（ORF）which from 119 to 832 nucleotides encoding 237 amino acids withmolecular weight 25 358.8 D，instability index is 21.6 and aliphatic index is 99.11，grand average ofhydropathicity is 0.078. Blast alignment analysis indicated highly homologous to Ts2 gene especially forcucumber which has 96% homologous relationship，pathway analysis indicated CmTs2 gene located theupstream of gibberellin synthesis pathway. Real time-PCR analysis indicated that the expression level of CmTs2 was highest expressed in andromonecious but lowest in gynoecious in leaf and flower，which showed the CmTs2 gene might involve in the flower development female flower.