ZNF300促进巨核细胞分化

Objective：To study the function of ZNF300 in the megakaryocytes differentiation and proliferation. Methods: Public data analysis of ZNF300 expression and megakaryocyte culture to reveal the association of ZNF300 expression with leukemia and megakaryocyte differetniation; ZNF300 overexpression by lentiviral or retroviral infection and measure measure megakryocyte differentiation and proliferation by flowcytometry, MTT assay, and colony-forming unit assay; test ZNF300 subcellular localization by seprating cytosolic and nuclear extracts for Western Blotting. dual-luciferase assay and ChIP-qPCR to study ZNF300 target gene. Result: ZNF300 expression upregulation correlated with megakaryoyte differentiation;over-expression of ZNF300 promoted CD41 and CD61 expression, inhibited cell cycle progress, and reduced colony-forming unit. ZNF300 locolized in nuclear and regulated c-myc expression. Conclusion: ZNF300 promotes megakaryocytes differentiation.