miR-146a对类风湿关节炎患者滑膜成纤维细胞抑制作用的研究

Objective：To explore the effect of miR-146a on the proliferation and the IL-8 and IL-6 secretion of rheumatoid arthritis synovial fibroblasts(RASFs)。Methods：miR-146a was transfected into cultured synovial cells from RA patients in vitro by Lipofectamine2000. Fibroblast proliferation was assayed by 3H-TdR incorporation test; IL-6 and IL-8 in the cell culture supernatant were detected by EL ISA kit; mRNA expression of TRAF6 and IRAK1, the possible targets of miR-146a were detected by real-time PCR. Results: It is shown that the proliferation of RASFs transfected with miR-146a is obviously downregulated compared to those transfected with the negative control (p<0.01). Furthermore, the levels of IL-8 and IL-6 secretion are significantly lower in RASFs transfected with miR-146a than those with the negative control(p<0.01 and p<0.05). It is shown that the mRNA expression of IRAK1 is downregulated significantly by miR-146a transfection in RASFs(p<0.01). Conclusion: miR-146a could inhibit the proliferation and the secretion of IL-8 and IL-6 of RA synovial fibroblasts and IRAK1 might the main target of miR-146a resulting in the inhibitory effect in RASFs.