SFRP4和DKK1在宫颈鳞癌中的表达及临床病理意义
目的研究分泌型卷曲相关蛋白（SFRP4)及Wnt信号通路抑制因子DKK1（Dickkopf-1）在宫颈鳞癌中的表达及临床病理意义。方法 采用逆转录聚合酶链式反应(RT-PCR)检测66例宫颈鳞癌组织中及26例正常宫颈组织中DKK1和SFRP4 mRNA的表达水平；采用免疫组织化学法检测66例宫颈鳞癌组织中及26例正常宫颈组织中DKK1和SFRP4蛋白的表达水平。应用SPSS17.0统计学软件进行统计学分析，研究DKK1和SFRP4的表达与宫颈鳞癌患者主要临床病理指标的关系。结果 宫颈鳞癌SFRP4表达高于正常宫颈（P<0.01）。宫颈鳞癌DKK1表达低于正常宫颈（P<0.05）。DKK1和SFRP4在宫颈鳞癌中的表达与临床病理分期、组织分化程度、有无淋巴转移及浸润深度有关（P均<0.05）。DKK1与SFRP4在宫颈鳞癌中的表达呈负相关（P<0.01）。结论 Wnt信号通路上游因子SFRP4和DKK1在宫颈鳞癌发生中起关键作用，并且与宫颈鳞癌的临床病理指标密切相关。
Objective To investigate the expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and explore the clinicopathological implications. Methods Immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expressions of SFRP4 and DKK1 in 66 cervical squamous cell carcinoma and 26 normal cervical specimens. Results SFRP4 expression was significantly higher (P<0.01) and DKK1 expression was significantly lower (P<0.05) in the carcinoma tissues than in normal cervical tissues. DKK1 was negatively correlated with SFRP4 in the carcinoma tissues (P<0.01), and their expressions were associated with the clinical stages, tumor differentiation, depth of invasion and lymph node metastasis of the tumors (P<0.05). Conclusion SFRP4 and DKK1, the upstream components of the Wnt pathway, play a key role in the tumorigenesis of cervical squamous cell carcinoma, and their expressions are associated with the clinicopathological features of the malignancy.
侯安丽、李秀芬、李振环、方淑芹、张雅丽、徐亚威
肿瘤学基础医学妇产科学
宫颈鳞癌KK1SFRP4免疫组织化学法逆转录聚合酶链式反应
侯安丽,李秀芬,李振环,方淑芹,张雅丽,徐亚威.SFRP4和DKK1在宫颈鳞癌中的表达及临床病理意义[EB/OL].(2017-12-07)[2025-08-02].https://chinaxiv.org/abs/201712.00836.点此复制
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