响应面法优化蟹糊微生物抑制条件
Optimization of the Experimental Conditions Inhibit Microbial in Crab Paste by Response Surface Methodology
利用SAS中响应面分析方法，对茶多酚等防腐剂在蟹糊中微生物抑制作用进行研究，以菌落总数为响应值作响应面和等高线图。结果表明，初始菌数为2100cfu/g的蟹糊，放置于-12℃～-15℃下贮藏，7d后观察，经过优化后，抑制其菌落总数的最佳条件为0.38%的茶多酚，0.06%的乳酸链球菌素和0.03%的溶菌酶，此时菌落总数最小值约为3500cfu/g。并对优化条件做验证试验，得到同样条件下菌落总数为3600cfu/g，与预测值接近，表明模型拟合度好。
his paper is a study in inhibiting microbial in crab paste by response surface methodology,such as tea polyphenol,and three-dimensional plot and corresponding contour plot in response to total bacterial count are made.The results show that the best conditions in inhibiting are 0.38%tea polyphenol.0.06%Nisin and 0.03% lysozyme.and minimum number is 3500cfu/g.After 7 days\\\
李虹、娄永江
微生物学生物工程学生物科学研究方法、生物科学研究技术
茶多酚乳酸链球菌素溶菌酶响应面
ea polyphenolNisinlysozymeResponse Surface
李虹,娄永江.响应面法优化蟹糊微生物抑制条件[EB/OL].(2008-06-16)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/200806-328.点此复制
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