齐墩果酸对阿尔茨海默病模型大鼠海马区ADAM10、sAPPα及凋亡相关蛋白bax和bcl-2表达的影响

Objective:To observe the effect of oleanolic acid (OA ) on α-secretase (ADAM10), soluble amyloid precursor protein (sAPPα), and apoptosis-related protein bax, bcl-2 in hippocampus of rat models of Alzheimer's disease, to preliminary explore the possible mechanism of oleanolic acid treating AD. Methods: 30 male SD rats were randomly divided into sham group, model group and oleanolic acid group, and aggregation β- amyloid fragment 25-35 (Aβ25-35) was injected into the right lateral ventricle. After successfully build the AD model, feeding the rats in OA group with oleanolic acid in accordance with 20mg o Kg-1, and rats in sham group and model group were given the same volume of distilled water, once / day, for 4 weeks. Western blot was applied to detect ADAM10, sAPPα, bax, and bcl2. Results: Compared with sham group, ADAM10, sAPPα, bcl-2 expression in model group hippocampus was significantly decreased, and the difference was statistically significant (P <0.05); with significantly increased bax expression, and the difference was statistically significant (P <0.05); compared with the model group, ADAM10, sAPPα, bcl-2 protein expression was significantly increased in oleanolic acid group, and the difference was statistically significant (P <0.05); the expression of bax decreased significantly, and the difference was statistically significance (P <0.05). Conclusions: The ingredient in Fruits of Ligustrum lucidum, an herb for tonifying kidney, oleanolic acid could promote the expression of ADAM10, sAPPα, bcl-2, and inhibit the expression of bax in hippocampus of AD model rats to achieve a neuroprotective effect.?????