'王族'海棠MRYLC基因克隆与表达分析
loning and Expression analysis ofa MRYLCgene from Malus 'Royalty'
本研究从'王族'海棠中获得了编码bHLH转录因子的全长cDNA序列，命名为MRYLC。生物信息学分析结果表明，该序列编码区域共1956bp，编码651个氨基酸，其蛋白质相对分子质量为72.9kDa，理论等电点为5.51，位于细胞核的可能性最大。采用实时荧光定量研究了不同发育时期的MRYLC基因的表达水平，利用高效液相色谱分析色素变化情况，结果显示，随着叶片发育，MRYLC基因的表达量逐渐降低，与叶片中呈色色素的含量变化相关。
he full-length cDNA of abasic helix-loop-helix (bHLH) gene，which was named as MRYLC，was isolated from Malus'Royalty'. Bioinformatics analysis indicated that the MRYLC gene contains 1956-base pair(bp) open reading frame (ORF) and encodes a 651-amino-acid polypeptide with a predicted molecular weight of 72.9kDa and pI of 5.51,，has the most possibility located in cell nucleus. Real-time PCR analysis indicated that with leaves development, MRYLC expression level gradually reduced, with associated flavonoids changing in leaves ,which indicated by HPLC. .
王冰洁、李厚华、李果
分子生物学植物学遗传学
'王族'海棠MRYLC表达分析
Malus 'Royalty'MRYLCexpression
王冰洁,李厚华,李果.'王族'海棠MRYLC基因克隆与表达分析[EB/OL].(2016-06-02)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201606-165.点此复制
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