NIK在TNFα诱导NF-κB活化的过程中是必需的

In the cell, some proteins modified by ubiquitin play central role in regulating the NF-κB signaling pathway. The purpose is to study whether TNIK is necessary in NF-κB signaling pathway. By combining western blot and coimmunoprecipitation assays, we found that TNIK underwent ubiquitin- and proteasome-mediated degradation in response to TNFα. Further investigation through RNA interference, western blot and reporter activity assay disclosed that TNIK was necessary for NF-κB activation by TNFα. In conclusion, the finding indicated TNIK was necessary for NF-κB activation by TNFα.