OsMYB调控化感水稻酚酸类物质合成及其抑草作用研究

Regulation of gene expression is a vital process in the formation of rice allelopathy. In this study, allelopathic rice PI312777(Oryza sativa L.) was modified by RNA interference(RNAi) and overexpression(OE) technologies to inhibit or increase gene expression of OsMYB(CT829537) in PI312777 respectively. The results showed that up-regulation of phenolic synthesis related genes was found in CT829537-OE transgenic PI312777 co-cultured with barnyardgrass(BYG), compared with that of wild type. However, the reverse was true in the CT829537-RNAi transgenic PI312777. Up-regulation of the gene expression in CT829537-OE transgenic PI312777 increased phenolic acids contents in rice root and root exudates, which led to enhance allelopathic inhibition on barnyardgrass. In contrast, decrease of phenolic acids contents and weed inhibition was found in CT829537-RNAi transgenic PI312777. These results implied that CT829537 was responsible for regulating phenolic synthesis in allelopathic rice PI312777 and then enhanced allelopathic inhibition on weeds.