烟草配子膜融合后精核迁移依赖于微丝而不依赖于微管
Migration of sperm nuclear after gametes membrane fusion in tobacco depends on actin but not microtubule
被子植物受精过程中的一个重要事件是受精后雄核和雌核的会合，但是对这一问题的研究仍然是一片空白。本文以烟草为材料，用体外受精系统研究了受精后雄核和雌核的会合。与动物中雌雄核相向移动会合不同，被子植物中受精后，雌核保持在原位，雄核向雌核迁移以实现两者的会合。为了研究精核迁移的分子机理，用细胞骨架的特异性抑制剂进行了处理。结果表明精核向雌核迁移是一个依赖于微丝而不依赖于微管的过程。为了研究驱动精核迁移的力量来源，使用肌球蛋白的特异性抑制剂2,3-butanedione monoxime (BDM)进行了进一步的研究。在20 mM的浓度下，BDM并不能抑制精核的迁移，而且精核迁移的速度相对于对照没有明显的变化。这就强烈暗示另一种基于微丝的驱动力量，即肌动蛋白相关蛋白复合体（arp2/3 complex）参与了精核的迁移。
he cellular mechanisms underlying the migration of male nucleus and female nucleus are not yet understood in angiosperms. The gametes of tobacco were used to study the issue by fertilization in vitro. Contrary to the situation in animal cells, which male and female pronucleaar move to each other, sperm nuclear migrate to female nuclear. Inhibitors of cytoskeleton were used to investigate whether actin or microtubule is responding for the migration of SN and FN. The results indicated that the SN migration was actin and not microtubule dependent. Further the inhibitors of myosin BDM was used and show no evident effects on sperm nuclear migration. The result imply arp2/3 complex but not myosin motors was probobaly involved in regulating SN migration.
彭雄波
细胞生物学分子生物学植物学
微丝配子精核迁移
microfilamentgametesmigration of sperm nuclear
彭雄波.烟草配子膜融合后精核迁移依赖于微丝而不依赖于微管[EB/OL].(2011-01-27)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201101-1338.点此复制
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