低温对两种紫草科植物△6-脂肪酸脱氢酶基因表达的影响
Low temperature regulate △6-fatty acid desaturases at a transcriptional level in Arnebia euchroma and Nonea capsica(willd.) G. Don
【目的】研究两种紫草科植物新疆软紫草（Arnebia euchroma）和假狼紫草（Nonea capsica(willd.) G. Don）中γ-亚麻酸合成关键酶基因△6-脂肪酸脱氢酶基因的表达特性。【方法】以翻译延伸因子EF1a为内参基因，利用半定量逆转录聚合酶链式反应(semi-QRT-PCR)技术研究△6-脂肪酸脱氢酶基因的表达特性；用气相色谱技术分析产物γ-亚麻酸在低温下的积累情况。【结论】△6-脂肪酸脱氢酶基因在紫草植物的叶，茎，根中均表达，在低温诱导不同时间后紫草植物叶片中△6-脂肪酸脱氢酶基因的表达有所增强；产物γ-亚麻酸在低温下积累，这表明紫草科植物叶片中△6-脂肪酸脱氢酶基因表达量的增加可能与适应低温胁迫的逆境有关。
expression analysis of the △6-fatty acid desaturases gene, was carried out by Semi-QRT-PCR，using EF1a gene as reference gene, Semi-QRT-PCR results show that △6-fatty acid desaturases gene was expressed in leaves, stem and root of Arnebia euchroma and Nonea capsica (willd.) G. Don, The transcribing of △6-fatty acid desaturases gene in leaves were slightly enhanced by Low temperature．And the product γ-linolenic acid（GLA）accumulated in the leave under low temperature by gas chromatography analysis．These suggested that expression changed of △6-fatty acid desaturases may be related to helping the plants adapt to low temperature.
李冠、陈光辉、张芳转、周晶
生物化学分子生物学植物学
低温△6-脂肪酸脱氢酶γ-亚麻酸表达分析
kLow temperature△6-fatty acid desaturasesγ-linolenic acidExpression analysis
李冠,陈光辉,张芳转,周晶.低温对两种紫草科植物△6-脂肪酸脱氢酶基因表达的影响[EB/OL].(2011-07-26)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201107-386.点此复制
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