蛇床子素舒张大鼠离体肺动脉环作用机制
he Mechanisms of Osthole on relaxing isolated rat pulmonary arteries
目的：研究蛇床子素对大鼠离体预收缩肺动脉环舒张作用的具体机制。 方法：游离健康SD大鼠的肺动脉血管,剪成长约3mm的血管环，以苯肾上腺素（1μM）预收缩后，探讨去除血管内皮、孵育eNOS抑制剂L-NAME、钾通道抑制剂4-AP、glibenclamide、TEA、BaCl2后，不同浓度蛇床子素对PE预收缩肺动脉张力的影响。结果：1.去除血管内皮及孵育L-NAME均能降低蛇床子素的舒血管效应。2、4-AP（1mM）能降低蛇床子素舒血管效应，而glibenclamide (10 μM)、TEA (10 mM)、BaCl2(100 μM)对蛇床子素舒张预收缩大鼠离体肺动脉环的作用无明显影响。结论：蛇床子素通过促进内皮NO释放及开放Kv通道舒张大鼠离体肺动脉环。
Objective To study the underlying mechanism of Osthole on relaxing isolated rat pulmonary arteries by using force-electricity transducers. Methods The arteries are isolated from healthy SD rats, cut into rings (about 3mm in length) and precontracted by phenylephrine(PE,1μM),observed the effects of endothelium denuding, adding eNOS inhibitor or potassium channels inhibitors 4-AP, glibenclamide , TEA, BaCl2 on Osthole's relaxation effect on PE-induced artery contraction. Results 1. Endothelium-denuding and L-NAME inhibits the relaxation of Osthole on pulmonary arteries.3. We also observed the effect of Osthole on the contraction of rings induced by PE was attenuated in the presence of 4-AP（1mM）, while glibenclamide (10 μM)、TEA (10 mM)、BaCl2 (100 μM) have no effect. Conclusion Osthole relaxes isolated rat pulmonary arteries, which is related to the endothelium production of NO and openning Kv channels.
滕振霞、何观虹、姚丽
基础医学生理学药学
生药学蛇床子素肺动脉高压肺动脉环内皮钾通道
PharmacognosyOstholePulmonary arterial hypertensionPulmonary arterial ringEndotheliumK+ channels
滕振霞,何观虹,姚丽.蛇床子素舒张大鼠离体肺动脉环作用机制[EB/OL].(2014-12-17)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201412-515.点此复制
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