骨髓基质干细胞对过继免疫实验性自身免疫性脑脊髓炎的影响及免疫调节机制
Study on treatment of adoptive transfer experimental autoimmune encephalomyelitis by bone marrow stromal cells
探讨骨髓基质干细胞(BMSCs) 对过继免疫实验性自身免疫性脑脊髓炎(AT-EAE) 的治疗作用及相关免疫调节机制。分离纯化培养BMSCs ，建立EAE 动物模型，在EAE动物模型发病高峰期取淋巴细胞，加入MBP刺激并与BMSCs共培养，并设未共培养的单纯MBP刺激对照组。将培养后的淋巴细胞对大鼠进行过继免疫回输，通过体外增殖实验，流式细胞仪检测以及体内临床评分评估BMSCs对EAE的治疗作用及其机制。BMSCs共培养MBP 组与MBP 组相比，淋巴细胞增殖明显被抑制，CD4+Foxp3+细胞显著增高而CD4+IL-17+细胞显著降低，培养后的淋巴细胞进行过继免疫回输后，干细胞共培养组临床评分显著降低( P < 0. 05) 。BMSCs通过调节免疫系统的CD4 + Foxp3+ T细胞数和CD4+IL-17+细胞数即调节性T细胞（Treg）和Th17的细胞平衡，对EAE起治疗作用。
o investigate therapeutic effect and immunomodulation mechanism of bone marrow stem cells (BMSCs) treating adoptive transfer experimental autoimmune encephalomyelitis (AT-EAE) . Methods: To purify and culture BMSCs, and to establish EAE model , T lymphocytes were isolated at the peak of disease. T cells were stimulated with MBP in the present or absent of BMSCs, these cells were injected into the naive rat tail vein. To evaluate the corresponding indexes of EAE by Lymphocyte proliferation assays and Flow cytometry test in vitro, clinical assessment in vivo. Results: Compared with MBP group, the proliferation of lymphocytes and clinical score of (BMSCs + MBP) group significantly decreased ( P < 0. 05) . The proportion of Treg increased while Th17 decreased. Conclusion: BMSCs strongly reduces proliferative responses of Lymphocytes of EAE rats, promote Treg generation and inhibit Th17 cell generation.
王菁华、孔庆飞、刘玉梅、金连弘、孙博、刘希君、王广友、李呼伦、王丹丹
基础医学神经病学、精神病学
过继免疫EAE骨髓基质干细胞调节性T细胞h17
experimental autoimmune encephalomyelitisbone marrow stromal cellsregh17
王菁华,孔庆飞,刘玉梅,金连弘,孙博,刘希君,王广友,李呼伦,王丹丹.骨髓基质干细胞对过继免疫实验性自身免疫性脑脊髓炎的影响及免疫调节机制[EB/OL].(2009-01-13)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200901-554.点此复制
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