乙烯和伤害处理对番茄ACC氧化酶基因表达的影响
Expression Analysis of ACC Oxidase Genes of Tomato (Solanum Lycopersicon Mill.) by ethylene and wounding
本研究采用RT-PCR方法从野生型番茄中, 扩增出6个ACO基因家族成员的cDNA片段，采用半定量RT-PCR方法检测ACO基因家族各成员在番茄不同组织及果实不同发育时期的表达，同时分析了ACO家族基因在果实乙烯处理和叶伤害处理后的表达变化。研究结果表明：ACO各基因具有明显的组织特异性和时空表达特异性；乙烯诱导SlACO1、SlACO3、SlACO6的表达，抑制SlACO4的表达, 而对SlACO2、SlACO5基因的表达无明显影响；伤害处理诱导SlACO1、SlACO3、SlACO4的表达，对SlACO2、 SlACO5、SlACO6的表达无显著影响。这些结果为进一步明确ACO基因家族各成员的功能，改良番茄的耐贮性和抗逆性提供了理论依据。
In this studdy, six cDNA fragments of ACC oxidase gene family were obtained by RT-PCR from tomato (Solanum lycopersicon Mill. cv Ailsa Craig，AC++). The expression patterns of the genes were examined in various tissues and different stages of fruits by semi-quantitative RT-PCR. The expression of ACC oxidase genes in fruits and leaves was also analyzed after ethylene and wounding treatment. The results showed that ACO genes have different spatial and temporal expression profiles in tomato; SlACO1, SlACO3 and SlACO6 genes were siganificantly induced by ethylene while SlACO4 gene has opposite result. Moreover, the expression of SlACO1, SlACO4 and SlACO3 was increased after wounding treatment. These results provided a set of important data for exploring the functions of ACO genes and improving the storability and the resistance of tomato.
胡宗利、陈国平、解巧利、董婷婷、李栀恩、朱明库、张彦杰
分子生物学植物学遗传学
番茄氧化酶基因半定量RT-PCR乙烯和伤害处理
tomatoACC Oxidase GeneSemi-quantitative PCREthylene and Wounding Treatment
胡宗利,陈国平,解巧利,董婷婷,李栀恩,朱明库,张彦杰.乙烯和伤害处理对番茄ACC氧化酶基因表达的影响[EB/OL].(2013-12-25)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/201312-831.点此复制
评论