番茄ANT基因家族的全基因组鉴定、结构特征及表达分析

NT(AINTEGUMENTA) gene family is a member of the AP2 subfamily witch is in the AP2/ERF family of transcription factors in plant. ANT gene family involves extensively in regulation of plant organs development and playing an important role in Auxin related signaling pathways. In order to study potential function of ANT gene family members in tomato, we investigated ANT family genes with the whole genome database using bioinformatics method. We did analysis of the structure characteristics, phylogenetic relationships, chromosomal location, and tissue expression pattern. The results showed that total 5 members were identified and they distributed in 5 chromosomes of tomato. They all included 2 AP2 conservative structure domain, 4 specific motifs and unique linker-region. Phylogenetic analysis indicated that tomato ANT transcription factors and the homologous gene in arabidopsis belonged to the euANT branch family of AP2 / ERF family, which suggested that they may have a similar origin. Quantitative PCR analysis indicated that all of the ANT genes showed expression in multiple plant tissues, with higher levels of expression in young tissues as compared with older tissues. ANT genes were low in stem and leaf tissues; mainly expressed in flower organ (Bud and flower); detected in immature fruit while very low in mature green, break, yellow fruit and red fruit stage.